International Journal of Experimental Dental Science

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VOLUME 2 , ISSUE 2 ( July-December, 2013 ) > List of Articles

RESEARCH ARTICLE

Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma

Tiago Novaes Pinheiro, Alberto Consolaro, Francisco Carlos Ribeiro

Citation Information : Pinheiro TN, Consolaro A, Ribeiro FC. Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma. Int J Experiment Dent Sci 2013; 2 (2):86-91.

DOI: 10.5005/jp-journals-10029-1047

Published Online: 00-12-2013

Copyright Statement:  Copyright © 2013; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

To evaluate the distribution of bacteria in teeth with pulp necrosis and apical granuloma.

Materials and methods

Thirty-two extracted teeth with pulp necrosis and apical granulomas and 16 cases of isolated apical granulomas were sectioned and stained with Hematoxylin and Eosin (H&E) and Brown-Brenn staining for microscopic evaluation. The specimens were observed as to the location and distribution of bacteria involved in the process of pulp necrosis and their relationship with the periapical tooth structures and soft tissues.

Results

Stainable Gram-positive and Gram-negative bacteria were found in the entire root canal system. Bacterial biofilms were detected in 81.2% of cementum root canal, 46.8% in apical delta, 65.6% at the apical surface and 79.2% of the granulomas above all the specimens. Bacteria and bacterial biofilms were present in the entire root canal system of the infected teeth with apical granulomas.

Conclusion

Our findings suggest that bacterial infections can reach inaccessible areas to instrumentation.

How to cite this article

Ribeiro FC, Consolaro A, Pinheiro TN. Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma. Int J Experiment Dent Sci 2013;2(2):86-91.


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