International Journal of Experimental Dental Science

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VOLUME 2 , ISSUE 2 ( July-December, 2013 ) > List of Articles


Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma

Tiago Novaes Pinheiro, Alberto Consolaro, Francisco Carlos Ribeiro

Citation Information : Pinheiro TN, Consolaro A, Ribeiro FC. Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma. Int J Experiment Dent Sci 2013; 2 (2):86-91.

DOI: 10.5005/jp-journals-10029-1047

Published Online: 01-12-2015

Copyright Statement:  Copyright © 2013; The Author(s).



To evaluate the distribution of bacteria in teeth with pulp necrosis and apical granuloma.

Materials and methods

Thirty-two extracted teeth with pulp necrosis and apical granulomas and 16 cases of isolated apical granulomas were sectioned and stained with Hematoxylin and Eosin (H&E) and Brown-Brenn staining for microscopic evaluation. The specimens were observed as to the location and distribution of bacteria involved in the process of pulp necrosis and their relationship with the periapical tooth structures and soft tissues.


Stainable Gram-positive and Gram-negative bacteria were found in the entire root canal system. Bacterial biofilms were detected in 81.2% of cementum root canal, 46.8% in apical delta, 65.6% at the apical surface and 79.2% of the granulomas above all the specimens. Bacteria and bacterial biofilms were present in the entire root canal system of the infected teeth with apical granulomas.


Our findings suggest that bacterial infections can reach inaccessible areas to instrumentation.

How to cite this article

Ribeiro FC, Consolaro A, Pinheiro TN. Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma. Int J Experiment Dent Sci 2013;2(2):86-91.

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  1. Microbial biofilms. New York: Cambridge University Press 1995.p.15-301.
  2. Light and electron microscopic studies of root canal flora and periapical lesions. J Endod 1987;13:29-39.
  3. The effects of surgical exposure of dental pulps in germfree and conventional rats. Oral Surg Oral Med Oral Pathol 1965;20:340-349.
  4. Root resorption-etiology, terminology and clinical manifestations. Endod Dent Traumatol 1988;4:241-252.
  5. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Scand J Dent Res 1981;89:321-328.
  6. Coronal leakage as a cause of failure in root canal therapy: a review. Endod Dent Traumatol 1994;10:105-108.
  7. Endodontic leakage studies reconsidered. Part I. Methodology, application and relevance. Int Endod J 1993;26:37-43.
  8. Single-visit endodontics: a clinical study. J Endod 1983;9:147-152.
  9. A comparative study of the single-visit and the multiple-visit endodontic procedure. J Endod 1978;4:278-281.
  10. Evaluating success and failure. In: Walton RE, Torabinejad M, editors. Principles and practice of endodontics. 2nd ed. Philadelphia: WB Saunders; 1996; p.324-335.
  11. Healing of periapical lesions of pulpless teeth after endodontic treatment with controlled asepsis. Endod Dent Traumatol 1987;3:58-63.
  12. Clinical, radiographic and histologic study of endodontic treatment failures. Oral Surg Oral Med Oral Pathol 1991;71: 603-611.
  13. Regional variation in root dentinal tubule infection by Streptococcus gordonii. J Endod 1996;22:290-293.
  14. A method for the differential staining of Gram-positive and Gram-negative bacteria in tissue sections. Bull Johns Hopkins Hosp 1931;48:69-73.
  15. Bacterial status in root-filled teeth exposed to the oral environment by loss of restoration and fracture or caries: a histobacteriological study of treated cases. Int Endod J 2003;36:787-802.
  16. Bacterial invasion of pulpal dentin wall in vitro. J Dent Res 1982;61:435-438.
  17. Cemento enamel junction: microscopic analysis and external cervical resorption. J Endod 2000;26: 503-508.
  18. A scanning electron microscopic evaluation of in vitro dentinal tubules penetration by selected anaerobic bacteria. J Endod 1996;22:308-310.
  19. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after one-visit endodontic treatment. Oral Surg Oral Med Oral Pathol 2005;99:231-252.
  20. Treponema socranskii in primary endodontic infections as detected by nested PCR. J Endod 2003;29:244-247.
  21. Periapical bacterial plaque in teeth refractory to endodontic treatment. Endod Dent Traumatol 1990;6:73-77.
  22. Microbiological monitoring and results of treatment of extraradicular endodontic infection. Endod Dent Traumatol 1990;6:129-136.
  23. A comparison of rootcanal and apical lesion flora. Brit Dent J 1964;116:350-352.
  24. The evolving new understanding of endodontic infections. Endod Topics 2003;6:57-77.
  25. A histopathologic and histobacteriologic study of 35 periapical endodontic surgical specimens. J Endod 1977;3:8-23.
  26. Effect of histological decalcifying agents on number and stainability of Gram-positive bacteria. J Dent Res 1987;66:1029-1031.
  27. An immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions. J Endod 2003;29:194-200.
  28. The role of oral microorganisms in the pathogenesis of periapical pathosis. I. Effect of Streptococcus mutans and its cellular constituents on the dental pulp and periapical tissue of cats. J Endod 1983;9:171-175.
  29. Biofilm: A dental microbial infection. J Nat Sc Biol Med 2011;2:71-75.
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