International Journal of Experimental Dental Science

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VOLUME 5 , ISSUE 1 ( January-June, 2016 ) > List of Articles


Microstrain of Human Root Dentin after Chelating Agents Application

Abdillah Imron Nasution

Citation Information : Nasution AI. Microstrain of Human Root Dentin after Chelating Agents Application. Int J Experiment Dent Sci 2016; 5 (1):11-15.

DOI: 10.5005/jp-journals-10029-1116

Published Online: 01-08-2010

Copyright Statement:  Copyright © 2016; The Author(s).



To determine the effect of ethylene diamine tetra acetic acid (EDTA) and RcPrep to microstrain of human root dentin.

Materials and method

Fifteen extracted human premolars were sectioned at the cementoenamel junction using a diamond bur disk. Every group was then sectioned horizontally in the third cervical, third middle and third apical to have total of 45 specimens. Based on the test solutions used, specimens were divided randomly into three groups: (1) the EDTA group: 0.05 ml of 17% EDTA for 15 minute (n = 15), (2) the RcPrep group, 0.05 ml of 15% EDTA-urea peroxide-carbowax for 15 minutes (n = 15), and (3) the control group, 1 ml of 0.9% saline for 15 minutes (n = 15). All specimens were prepared for the determination of microstrain of root dentin using X-ray diffraction (XRD) analysis. The microstrain of root dentin calculated using the equation ? = Br cos?/sin? then statistically analyzed using regression test.


Analysis of regression between control and treatment groups were significant (p < 0.05). The microstrain was significantly greater with 17% EDTA when compared with 15% RcPrep. In addition, based on XRD result, all specimens showed same patterns as apatite groups. The pattern of RcPrep group showed better material than EDTA group.


EDTA and RcPrep can contribute to improvements of microstrain of root dentin. In comparing these properties, the RcPrep showed lower effect than EDTA.

How to cite this article

Nasution AI. Microstrain of Human Root Dentin after Chelating Agents Application. Int J Experiment Dent Sci 2016;5(1):11-15.

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