International Journal of Experimental Dental Science

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VOLUME 5 , ISSUE 2 ( July-December, 2016 ) > List of Articles

RESEARCH ARTICLE

Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts

Thuy Anh Vu Pham, Hao TT Nguyen, My TN Nguyen, Van NL Trinh, Nga Y Tran, Lan TQ Ngo, Ha LB Tran

Citation Information : Pham TA, Nguyen HT, Nguyen MT, Trinh VN, Tran NY, Ngo LT, Tran HL. Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts. Int J Experiment Dent Sci 2016; 5 (2):83-88.

DOI: 10.5005/jp-journals-10029-1131

Published Online: 00-12-2016

Copyright Statement:  Copyright © 2016; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aims

Our study focused on the fabrication of platelet-rich fibrin (PRF) and evaluated its influences on cell behaviors, including proliferation and migration.

Materials and methods

Platelet-rich fibrin was prepared from human peripheral blood according to Choukroun's method without using nonanticoagulant and foreign factors for platelet activation. Platelet-rich fibrin architecture was studied by hematoxylin and eosin staining. The investigation of PRF effects on human gingival fibroblasts (hGFs) was conducted via PRF liquid extract. Cell proliferation was determined via the number of cells after a period of time incubated in PRF liquid extract. Influence of PRF liquid extract on the migration of hGFs was conducted via scratch wound healing assay.

Results

Histological staining reviewed the natural fibrin fiber matrix of PRF. Platelet-rich fibrin liquid extract promoted hGF proliferation after 7 days of cultivation. Human gingival fibroblast proliferation in PRF liquid extract was more superior than those cultured in complete medium. Platelet-rich fibrin was also found to be able to promote the migration of hGFs for up to 48 hours.

Conclusion

These results indicated that PRF is suitable to be used as autologous natural biomaterial in supporting wound healing and in further application in periodontitis treatments.

How to cite this article

Nguyen HTT, Nguyen MTN, Trinh VNL, Tran NY, Ngo LTQ, Pham TAV, Tran HLB. Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts. Int J Experiment Dent Sci 2016;5(2):83-88.


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