International Journal of Experimental Dental Science

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VOLUME 7 , ISSUE 2 ( July-December, 2018 ) > List of Articles

REVIEW ARTICLE

How has Tooth Manipulation been Conducted for Dental Pulp Stem Cells Isolation? A Scoping Review

Camila P Ferrúa, Cainá C do Amaral, Roberta Giorgi, Tiago Garcia, Fernanda Nedel

Keywords : Dental pulp stem cell, Manipulation, Scoping review, Stem cell,Dental pulp

Citation Information : Ferrúa CP, do Amaral CC, Giorgi R, Garcia T, Nedel F. How has Tooth Manipulation been Conducted for Dental Pulp Stem Cells Isolation? A Scoping Review. Int J Experiment Dent Sci 2018; 7 (2):98-135.

DOI: 10.5005/jp-journals-10029-1182

License: CC BY-NC 4.0

Published Online: 00-12-2018

Copyright Statement:  Copyright © 2018; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

The aim of this study was to realize a scoping review the literature to verify how tooth manipulation for dental pulp stem cells (DPSCs) isolation has been conducted and if a standard tooth, preparation protocol for DPSCs isolation exists. The electronic search was conducted without initial date restriction up to and including April 2014 in PubMed, Scopus, Scielo, and ISI Web of Knowledge databases to identify studies that described the methodology used for DPSCs isolation. Two hundred and twenty-two articles were included and the information analysis was performed concerning dental manipulation and pulp tissue processing. Furthermore, the quality of included studies was evaluated through the assessment of the risk of bias. This scoping review established a platform for dental manipulation protocols for DPSCs isolation purposes. Over the past years, many studies have been conducted using DPSCs. However, there is a clear lack of standardization in tooth manipulation before DPSCs isolation. Currently, given a large number of variables in cell isolation techniques and all possible consequences in the in vitro behavior of cells, it is important to reinforce the importance of standard protocols to obtain a uniform cell culture.


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