International Journal of Experimental Dental Science

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VOLUME 9 , ISSUE 2 ( July-December, 2020 ) > List of Articles

RESEARCH ARTICLE

Assessment of Karyorrhexis Incidence in Exfoliated Buccal Mucosa Epithelial Cells among Fuel Station Employees in Sleman, Special Region of Yogyakarta, Indonesia

Regina TC Tandelilin, Tang Sze Mun, Tetiana Haniastuti

Keywords : Benzene, Buccal cells, Fuel station employees, Karyorrhexis incidence

Citation Information : Tandelilin RT, Mun TS, Haniastuti T. Assessment of Karyorrhexis Incidence in Exfoliated Buccal Mucosa Epithelial Cells among Fuel Station Employees in Sleman, Special Region of Yogyakarta, Indonesia. Int J Experiment Dent Sci 2020; 9 (2):62-69.

DOI: 10.5005/jp-journals-10029-1216

License: CC BY-NC 4.0

Published Online: 00-12-2020

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Introduction: Benzene exposure from petrol vapor possesses a health risk to humans, particularly fuel station employees through the inward breath of the unstable portions of petrol during vehicles refueling. This may trigger an increase in nuclear abnormalities and lead to DNA impairment pieces of evidence. The purpose of this study was to evaluate the incidence of karyorrhexis occurred in buccal mucosa epithelial cells among fuel station employees in Sleman, Special Region of Yogyakarta, Indonesia, to explore the possible cytogenetic risk on occupational exposure to petrol derivatives. Materials and methods: A total of 15 fuel station employees and 15 control subjects within the age group of 20–55 years were initiated. Buccal smears were obtained from the oral cavity with cytobrush and smeared into slides. Afterward, these specimens were stained with Papanicolaou\'s method and then analyzed for nuclear abnormalities. Karyorrhexis incidence was recorded as per 1,000 cells counted using a light microscope and then statistically analyzed with an independent T-test (p < 0.05). Results: The result revealed that there were statistically significant higher frequencies (p < 0.05) of karyorrhexis incidence between the exposed employees compared to the controls. The result revealed that among the exposed employees compared to the controls there were statistically significant higher frequencies (p < 0.05) of karyorrhexis incidence. Conclusion: This research concluded that exposure to petrol derivatives such as benzene increases the karyorrhexis incidence of exfoliated buccal mucosa epithelial cells on fuel station employees in Sleman, Special Region of Yogyakarta, Indonesia. Clinical significance: This study indicates increased karyorrhexis incidence of exfoliated buccal mucosa epithelial cells caused by repeated exposure to benzene.


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