International Journal of Experimental Dental Science

Register      Login

VOLUME 6 , ISSUE 2 ( July-December, 2017 ) > List of Articles

ORIGINAL ARTICLE

Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study

Abhinav C Singhal, Aditi S Jain, Jayesh Tiwari, Pratik Surana

Citation Information : Singhal AC, Jain AS, Tiwari J, Surana P. Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study. Int J Experiment Dent Sci 2017; 6 (2):65-67.

DOI: 10.5005/jp-journals-10029-1158

License: CC BY 3.0

Published Online: 01-12-2010

Copyright Statement:  Copyright © 2017; The Author(s).


Abstract

Introduction

The main objective of root canal treatment is to disinfect the entire root canal system, and irrigation is an important step in reducing the bacterial load from the root canal system. However, irrigants currently used in the field of endodontics have their share of limitations, and the search for an ideal root canal irrigant continues. The use of herbal extracts as endodontic irrigants is today gaining popularity.

Aims and objectives

To evaluate and compare the antimicrobial potential of herbal extracts, such as neem (Azadirachta indica), triphala (Terminalia chebula), green tea (Camellia sinensis), and combination of neem and triphala as endodontic irrigants against Enterococcus faecalis.

Materials and methods

Freshly prepared extracts of neem, triphala, green tea, and 2% chlorhexidine were used to assess the antimicrobial efficiency against E. faecalis using the agar well diffusion test. Agar plates were incubated at 370°C for 24 hours in an incubator. The diameter of bacterial inhibition zones around each well was recorded to the nearest size in mm.

Results

Higher mean zone of inhibition was recorded in chlorhexidine followed by neem extract and combination of neem and triphala than triphala and green tea extract respectively. The lowest mean zone of inhibition was found in green tea extract.

Conclusion

From the present study, it can be concluded that neem leaf extract shows comparable zones of inhibition with that of chlorhexidine and combination of neem and triphala.

How to cite this article

Singhal AC, Jain AS, Tiwari J, Surana P, Saini N. Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study. Int J Experiment Dent Sci 2017;6(2):65-67.


HTML PDF Share
  1. In vitro effectiveness of different endodontic irrigants on the reduction of Enterococcus faecalis in root canals. J Clin Exp Dent 2010 Jul;2:e169-e172.
  2. Comparative study of the antimicrobial efficiency of neem leaf extract, sodium hypochlorite and biopure MTAD — an in vitro study. Indian J Dent Adv 2012 Apr;4(1):740-743.
  3. Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. J Antimicrob Chemother 2008 Jun;61(6):1295-1301.
  4. Efficacy of passive ultrasonic irrigation with natural irrigants (Morinda citrifolia juice, Aloe Vera and Propolis) in comparison with 1% sodium hypochlorite for removal of E. faecalis biofilm: an in vitro study. Indian J Dent Res 2013 Jan-Feb;24(1):35-41.
  5. Microbial ecosystem analysis in root canal infections refractory to endodontic treatment. J Endod 2016 Aug;42(8):1239-1245.
  6. Discuss the role of microorganisms in the aetiology and pathogenesis of periapical disease. Aust Endod J 2016 Aug;42(2):53-59.
  7. In vivo quantitative evaluation of live and dead bacteria in root canal infection by using propidium monoazide with real-time PCR. J Endod 2013 Nov;39(11):1359-1363.
  8. Comparative safety of various intracanal irrigation systems. J Endod 2009 Apr;35(4):545-549.
  9. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006 Feb;32(2):93-98.
  10. Characterization of virulence factors and clonal diversity of Enterococcus faecalis isolates from treated dental root canals. Res Microbiol 2011 Feb-Mar;162(2):151-158.
  11. Comparative anti-microbial efficacy of Azadirachta indica irrigant with standard endodontic irrigants: a preliminary study. J Conserv Dent 2014 Mar;17(2):133-137.
  12. Comparison of antimicrobial efficacy of propolis, Morinda citrifolia, Azadirachta indica (Neem) and 5% sodium hypochlorite on Candida albicans biofilm formed on tooth substrate: an in vitro study. J Conserv Dent 2013 Nov;16(6):532-535.
  13. Reduction in Enterococcus faecalis counts—a comparison between rotary and reciprocating systems. Int Endod J 2014 Apr;47(4):380-386.
  14. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006 Feb;32(2):93-98.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.