International Journal of Experimental Dental Science

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VOLUME 4 , ISSUE 1 ( January-June, 2015 ) > List of Articles

RESEARCH ARTICLE

Cytotoxic Effects of Three Denture Base Materials on Gingival Epithelial Cells and Fibroblasts: An in vitro Study

Russell Wicks, Jegdish Babu, Franklin Garcia-Godoy, David Tipton

Citation Information : Wicks R, Babu J, Garcia-Godoy F, Tipton D. Cytotoxic Effects of Three Denture Base Materials on Gingival Epithelial Cells and Fibroblasts: An in vitro Study. Int J Experiment Dent Sci 2015; 4 (1):11-16.

DOI: 10.5005/jp-journals-10029-1088

Published Online: 01-06-2017

Copyright Statement:  Copyright © 2015; The Author(s).


Abstract

Objectives

Modern polyamide ‘flexible’ denture base materials have increased in popularity for use in removable partial dentures. The introduction of these new products warrants investigation of their relative potential for toxicity. The purpose of this study was to investigate three contemporary denture base materials used in fabricating definitive prosthetic restorations

Materials and methods

Two ‘flexible’ materials (Valplast™ and Duraflex™) formed by thermoplastic injection molding technique, and one traditional heat processed, methyl methacrylate resin material (Lucitone 199) were evaluated. Cultured gingival epithelial cells and fibroblasts were treated with conditioned media prepared from denture material disks and then assayed for cell toxicity by [3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide] (MTT) cell viability assay. Cell membrane damage was determined by measuring the release of cytoplasmic lactate dehydrogenase. Further confirmation of toxicity induced by the conditioned media was determined by staining the cells with live/dead stain and observing under a UV microscope.

Results

Data were analyzed by means of a linear model ANOVA followed by Tukey's post hoc tests for comparison among groups. The significance level adopted was 5% (p < 0.05). The three denture materials differed in their toxicity to the cells as assessed by MTT assay. Valplast conditioned media in general, especially the media of unpolished disks, was found to be toxic to both gingival fibroblasts and epithelial cells while media obtained from polished Lucitone and Duraflex were found to be less toxic. After 7 days of incubation with Valplast unpolished conditioned media, only 1 to 2% of the cells remained viable, while the polished disk conditioned media caused significantly less (p < 0.05) toxicity, approximately 76 and 92% of fibroblasts and epithelial cells respectively, were viable. After 7 days of incubation with media obtained from the other denture materials, 35 to 92% of fibroblasts and epithelial cells were found to be viable. The data obtained from lactate dehydrogenase (LDH) assay and live/dead mammalian cell viability assay were in agreement with the MTT viability assay.

Conclusion

Conditioned media from unpolished Valplast denture material appeared to be significantly more toxic to gingival fibroblasts and epithelial cells when compared to the polished Lucitone disk conditioned media as well as the media obtained from Duraflex.

How to cite this article

Ahuja S, Babu J, Wicks R, Garcia- Godoy F, Tipton D. Cytotoxic Effects of Three Denture Base Materials on Gingival Epithelial Cells and Fibroblasts: An in vitro Study. Int J Experiment Dent Sci 2015;4(1):11-16.


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  1. Denture base materials. Dent Clin North Am 1996;40(1):113-120.
  2. Cytotoxicity of denture base acrylic resins: a literature review. J Prosthet Dent 2003;90(2):190-193.
  3. The cytotoxic effect of denture base polymers. Acta Odontol Scand 1981;39(2):101-106.
  4. Leaching and cytotoxicity of formaldehyde and methyl methacrylate from acrylic resin denture base materials. J Prosthet Dent 1994;71(2):618-624.
  5. Relationships between denture base resin cytotoxicity and cell lipid metabolism. Int J Prosthodont 1995; 8(6):580.
  6. Cytotoxic effects of denture base materials on a permanent human oral epithelial cell line and on primary human oral fibroblasts in vitro. Int J Prosthodont 2001;14(5):439-443.
  7. Patterns of cell death induced by eluates from denture base acrylic resins in U-937 human monoblastoid cells. Eur J Oral Sci 2000;108(1):59-69.
  8. In vitro cytotoxicity of fibre-polymethyl methacrylate composite used in dentures. J Oral Rehabil 1999;26(8):666-671.
  9. Cytotoxicity of denture base resins. Int J Prosthodont 1997; 10(1):73-77.
  10. Cytotoxicity of denture base and hard chairside reline materials: a systematic review. J Prosthet Dent 2012;107(2): 114-127.
  11. Cytotoxicity of four types of resins used for removable denture bases: in vitro comparative analysis. Minerva Stomatol 2009;58(9):425-434.
  12. In vitro comparison of the cytotoxicity of acetal resin, heat-polymerized resin, and autopolymerized resin as denture base materials. J Biomed Mater Res B Appl Biomater 2009;91(2):905-909.
  13. Release of methyl methacrylate from heat-cured and autopolymerized resins: cytotoxicity testing related to residual monomer. Aust Dent J 1999;44(1):25-30.
  14. Biocompatibility of denture base acrylic resins evaluated in culture of L929 cells. Effect of polymerisation cycle and post-polymerisation treatments. Gerodontol 2007; 24(1):52-57.
  15. Effect of post-polymerization heat treatments on the cytotoxicity of two denture base acrylic resins. J Appl Oral Sci 2006;14(3):203-207.
  16. Cytotoxicity of denture base resins: effect of water bath and microwave postpolymerization heat treatments. Int J Prosthodont 2004;17(3):340-344.
  17. Survey of partial removable dental prosthesis (partial RDP) types in a distinct patient population. J Prosthet Dent 2011; 106(1):48-56.
  18. J Prosthet Dent 2005;94(1):11,36.
  19. An epithelial cell line grown in continuous culture from adult human gingiva. Proc Soc Exp Biol Med 1966;121(4):1294-1296.
  20. Karyotypic analysis of an epithelial-like cell line derived from normal adult human gingiva. Exp Cell Res 1966;43(3):633-638.
  21. Interleukin 1ß and tumor necrosis factor independent monocyte stimulation of fibroblast collagenase activity. J Cell Biochem 1990;44(4): 253-264.
  22. Cytotoxicity of sanguinarine chloride to cultured human cells from oral tissue. Pharmacoly Toxicol 1996;78(6): 397-403.
  23. Protection of ATP-depleted cells by impermeant strychnine derivatives. Implications for glycine cytoprotection. Am J Patholy 2001;158(3):1021-1028.
  24. Changes in roughness of denture base and reline materials by chemical disinfection or microwave irradiation: surface roughness of denture base and reline materials. J Appl Oral Sci 2011;19(5):521-528.
  25. Cytotoxicity of eluates from light-polymerized denture base resins. J Prosthet Dent 1994;72(6):644-650.
  26. Cytotoxicity of two autopolymerized acrylic resins used in orthodontics. Angle Orthod 2008;78(5): 926-930.
  27. Morphological analysis and interleukin release in human gingival fibroblasts seeded on different denture base acrylic resins. Int J Immunopathol Pharmacol 2012;25(3):637-643.
  28. Cytotoxicity of denture base acrylic resins: a literature review. J Prosthet Dent 2003;90(2):190-193.
  29. Long term biocompatibility test of denture base resins in vitro. J Prosthet Dent 1984;52(5): 694-699.
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