International Journal of Experimental Dental Science

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VOLUME 4 , ISSUE 2 ( July-December, 2015 ) > List of Articles


Comparison of Fungal Biofilm Formation on Three Contemporary Denture Base Materials

Russell Wicks, Jegdish Babu, Franklin Garcia-Godoy, Vinay Jain

Citation Information : Wicks R, Babu J, Garcia-Godoy F, Jain V. Comparison of Fungal Biofilm Formation on Three Contemporary Denture Base Materials. Int J Experiment Dent Sci 2015; 4 (2):104-108.

DOI: 10.5005/jp-journals-10029-1106

Published Online: 01-04-2010

Copyright Statement:  Copyright © 2015; The Author(s).



The purpose of this study was to investigate the potential of three denture base materials to support fungal biofilm formation.

Materials and methods

Specimens of two ‘flexible’ nylon type materials and one traditional heat processed, methyl methacrylate resin material were studied (both polished and unpolished surfaces). The specimens were coated with saliva and evaluated for fungal (Candida albicans) biofilm formation. The fungal biofilm mass formed on denture substrates were evaluated by dry weight analysis and by determining the number of viable fungal cells in the biofilm by MTT viability assay. Alteration in fungal metabolic function following the treatment of the biofilm C. albicans with nystatin and fluconazole was determined by XTT assay.


In general, the unpolished surfaces of the denture disks favored the fungal biofilm, the most being on polyamide specimen, Valplast. Significantly, less biofilm was formed on Duraflex and Lucitone surfaces. Biofim on C. albicans was also found to be resistant to antifungal agents. As compared to freshly incubated (grown) planktonic cells, biofilm fungal cells required significantly higher concentrations of nystatin and fluconazole in order to obtain 50% reduction in metabolic activity.


This study demonstrated the differences in denture materials to support fungal biofilm formation, and also difference between polished and unpolished denture material surfaces. The results demonstrated that one of the polyamide materials (duraflex) had lesser potential to biofilm formation than the others.

Clinical significance

Unfavorable tissue responses can ensue from the presence of fungal biofilms on dental prosthetics. Resistance to biofilm formation is a factor for dental materials in their selection and usage. This study helps to quantify, evaluate and compare biofilm formation on polished and unpolished surfaces of three commonly used denture base materials. The results of this study helped to identify materials, which may, therefore, be better indicated in clinical applications. Evaluations for the newer denture base materials, specific to these testing methods, appear to be novel in the scientific literature.

How to cite this article

Jain V, Babu J, Ahuja S, Wicks R, Garcia-Godoy F. Comparison of Fungal Biofilm Formation on Three Contemporary Denture Base Materials. Int J Experiment Dent Sci 2015;4(2):104-108.

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