International Journal of Experimental Dental Science

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VOLUME 5 , ISSUE 2 ( July-December, 2016 ) > List of Articles

RESEARCH ARTICLE

Influence of Additives to Bovine Bone Material in Osseous Regeneration of Mandibular Defect: An Animal Study using DXA

Franklin Garcia-Godoy, Mohammad Ali Saghiri, Jafar Orangi, Nader Tanideh, Armen Asatourian, Nader Sheibani

Citation Information : Garcia-Godoy F, Saghiri MA, Orangi J, Tanideh N, Asatourian A, Sheibani N. Influence of Additives to Bovine Bone Material in Osseous Regeneration of Mandibular Defect: An Animal Study using DXA. Int J Experiment Dent Sci 2016; 5 (2):104-108.

DOI: 10.5005/jp-journals-10029-1135

Published Online: 01-12-2010

Copyright Statement:  Copyright © 2016; The Author(s).


Abstract

Introduction

Increasing bone quality and quantity in the areas with insufficient bone volume is a major concern among scientists. Ideal bone substitute materials should have osteogenicity, osteoconductivity, and osteoinductivity. Clinoptilolite offers bovine deorganified crystalline bone materials, the advantage of being very similar to human bone with regard to its pore morphology and crystalline structure. This study evaluated the effect of adding Clinoptilolite to Bio-Oss on the osseous regeneration and bone healing process using serial dual-energy X-ray absorptiometry (DXA).

Materials and methods

A total of 64 rabbits were anesthetized and a bone defect was created on both semi-mandibles. The rabbits were divided into four equal groups: A (Bio-Oss®); B (Bio-Oss® with 2% Clinoptilolite mixture); C (allograft); and D receiving no treatment. The bone healing response of animals was tested after 2, 14, 30, and 60 days.

Results

Statistical analysis showed significant differences at time intervals before 14 days between allograft and other groups (p < 0.05). In all the defects filled with the tested materials, bone formation was observed subjectively. At 30-and 60-day intervals, there were no significant differences between allograft and Bio-Oss with 2% Clinoptilolite group (p = 0.052 and p = 0.260 respectively) although it was significant in 2-and 14-day intervals.

Conclusion

Clinoptilolite (2%) can be used to improve the osteoinduction property of bovine deorganified crystalline bone material. Clinoptilolite can be suggested as a potential material added to bone substitute materials due to its porous structure and buffering capacity and adsorption of a number of serum components which aids the osseous regeneration and healing process.

How to cite this article

Saghiri MA, Orangi J, Tanideh N, Asatourian A, Garcia-Godoy F, Sheibani N. Influence of Additives to Bovine Bone Material in Osseous Regeneration of Mandibular Defect: An Animal Study using DXA. Int J Experiment Dent Sci 2016;5(2):104-108.


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  1. Osteoinductive biomaterials— properties and relevance in bone repair. J Tissue Eng Regen Med 2007 Jan-Feb;1(1):25-32.
  2. Bone augmentation in rabbit calvariae: comparative study between Bio-Oss® and a novel ??TCP/DCPD granulate. J Clin Periodontol 2006 Dec;33(12):922-928.
  3. Effect of bioactive ceramic composition and structure on in vitro behavior. III. Porous versus dense ceramics. J Biomed Mater Res 1994 Nov;28:1303-1309.
  4. Effect of porosity and physicochemical properties on the stability, resorption, and strength of calcium phosphate ceramics. Ann NY Acad Sci 1988;523:227-233.
  5. Growth and adhesion of osteoblast-like cells derived from neonatal rat calvaria on calcium phosphate ceramics. J Biosci Bioeng 2000;89(1):18-26.
  6. Biomaterial developments for bone tissue engineering. Biomaterials 2000 Dec;21(23):2347-2359.
  7. Clinical evaluation of anorganic bovine bone xenograft with a bioabsorbable collagen barrier in the treatment of molar furcation defects. Int J Periodontics Restorative Dent 2001 Apr;21(2):161-169.
  8. Effect of porous xenographic bone graft with collagen barrier membrane on periodontal regeneration. Int J Periodontics Restorative Dent 2002 Aug;22(4):389-397.
  9. Maxillary sinus floor augmentation in rabbits: a comparative histologichistomorphometric study between rhBMP-2 and autogenous bone. Int J Periodontics Restorative Dent 2001 Jun;21(3):252-263.
  10. Histomorphometric study on healing of critical sized defect in rat calvaria using three different bovine grafts. J Tissue Eng Regen Med 2012 Oct;9(5):276-281.
  11. Periodontal regeneration with an autogenous bone-Bio-Oss composite graft and a Bio-Gide membrane. Int J Periodontics Restorative Dent 2001 Apr;21(2):109-120.
  12. Sinus lift grafts and endosseous implants. Treatment of the atrophic posterior maxilla. Dent Clin North Am 1992 Jan;36(1):151-186; discussion 87-88.
  13. Sinus grafting with porous bone mineral (Bio-Oss) for implant placement: a 5-year study on 15 patients. Int J Periodontics Restorative Dent 2000 Jun;20(3):245-253.
  14. Biocompatibility evaluation of Zeolite compared to bone HA, calcium phosphate (Ca2PO4) and eugenol paste. Key Eng Mater 2005 Apr;284-286:561-564.
  15. A sustainable approach for acid rock drainage treatment using clinoptilolite. EGU General Assembly Conference Abstracts 2009, Vienna, Austria; 2009. p. 1611.
  16. Intestinal development and function of broiler chickens on diets supplemented with clinoptilolite. AJAS 2013 Jul;26(7):987-294.
  17. Investigations on the synthesis and crystallization of hydroxyapatite at low temperature. Bio-Med Mater Eng 2004;14(4):581-592.
  18. Mechanical properties and in vitro cell compatibility of hydroxyapatite ceramics with graded pore structure. Biomaterials 2002 Nov;23(21):4285-4294.
  19. Bone cell proliferation on carbon nanotubes. Nano Lett 2006 Mar;6(3):562-567.
  20. Attachment and proliferation of osteoblasts and fibroblasts on biomaterials for orthopaedic use. Biomaterials 1995 Mar;16(4):287-295.
  21. Bone quality: what is it and how is it measured? Arq Bras Endocrinol Metab 2006 Aug;50(4):579-585.
  22. Validation and application of dual-energy X-ray absorptiometry to measure bone mineral density in rabbit vertebrae. J Clin Densitometry 2000 Spring;3(1):49-55.
  23. Accuracy and precision of bone mineral density and bone mineral content in excised rat humeri using fan beam dualenergy X-ray absorptiometry. Bone 2002 Jan;30(1):243-246.
  24. Biological evaluation of medical devices – Part 2: Animal welfare requirements; 2006.
  25. Dentistry – preclinical evaluation of biocompatibility of medical devices used in dentistry – test methods for dental materials; 2008.
  26. Effect of endodontic cement on bone mineral density using serial dual-energy x-ray absorptiometry. J Endod 2014 May;40(5):648-651.
  27. The use of porous ?-tricalcium phosphate blocks with platelet-rich plasma as an onlay bone graft biomaterial. J Periodontol 2007 Feb;78(2):315-321.
  28. Resorbability of bone substitute biomaterials by human osteoclasts. Biomaterials 2004 Aug;25(18):3963-3972.
  29. Bone augmentation potential in rabbit calvaria and ex vivo cytotoxicity of four bone substituting materials. J Periodont Implant Dent 2011;3(1):1-7.
  30. Therapeutics of stem cells in periodontal regeneration. J Nat Sc Biol Med 2011;2:38-42.
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