International Journal of Experimental Dental Science

Register      Login

VOLUME 6 , ISSUE 1 ( January-June, 2017 ) > List of Articles


The Effect of Periogen Solution on Dental Calculus in vitro: A Pilot Study

Marta Radnai, Zsolt Rajnics

Citation Information : Radnai M, Rajnics Z. The Effect of Periogen Solution on Dental Calculus in vitro: A Pilot Study. Int J Experiment Dent Sci 2017; 6 (1):33-34.

DOI: 10.5005/jp-journals-10029-1150

License: CC BY 3.0

Published Online: 01-04-2011

Copyright Statement:  Copyright © 2017; The Author(s).



Calculus can accommodate teeth and prosthetic restorations when the patient's oral hygiene is poor. Hardened calculus cannot be removed by patients, it needs professional cleaning using ultrasonic scaler or hand instrument. Solutions dissolving and preventing accumulation of dental calculus may help to keep dentures clean. The aim of this investigation was to examine the effect of Periogen on dental calculus in vitro.

Materials and methods

Calculus was collected via scaling from patients during a routine dental checkup. The samples were stored in carbamide peroxide solution (5%) for 24 hours, then rinsed and stored in distilled water. First, the Ca2+ content of the calculus was determined by photometric method after treating with cc. hydrochloric acid solution for 1 hour. The calculus samples were put in Periogen solution, prepared according to manufacturer's instruction for 16 hours, then crushed and put in Periogen solution with the same concentrate for 4.5 hours. The Ca2+ dissolved from calculus was measured using same photometric method.


Calculus samples contained 26 mg/100 mg Ca2+, which is similar to dentin (27–28 mg/100 mg) and to enamel (36 mg/100 mg). The Ca2+ dissolved from calculus after treating with cc. hydrochloric acid for 1 hour was considered 100%. Ca2+ dissolution was 1.5% after 16 hours (0.09%/h) and 5.45% (1.21%/h) for the next 4.5 hours after pulverizing the sample. This showed Periogen Ca2+ dissolution was 385 µg/100 mg after 16 hours direct contact with the material.


The experiment showed that pulverizing the previously hard calculus was done easily after soaking it in Periogen. The ability of Periogen to soften the calculus needs to be further investigated.

How to cite this article

Rajnics Z, Radnai M. The Effect of Periogen Solution on Dental Calculus in vitro: A Pilot Study. Int J Experiment Dent Sci 2017;6(1):33-34.

  1. Calculus removal and the prevention of its formation. Periodontol 2000 2011 Feb;55(1):167-188.
  2. Subgingival calculus: where are we now? A comparative review. J Dent 2000 Feb;28(2): 93-102.
  3. Dental calculus: a strategic review. Int J Dent Health Sci 2014;1(5):788-795.
  4. Clinical evaluation of removable partial dentures on the periodontal health of abutment teeth: a retrospective study. Open Dent J 2015 Mar;9:132-139.
  5. The effect of removable partial dentures on periodontal health of abutment and non-abutment teeth. J Periodontol 2002 Feb;73(2): 137-144.
  6. A microbiological evaluation of the use of denture cleansers in combination with an oral rinse in complete denture patients. Indian J Dent Res 2010 Jul-Sep;21(3):353-356.
  7. Investigation into the cleanliness of dentures in an elderly population. J Prosthet Dent 1990 Jul;64(1):48-52.
  8. Oral candidosis, denture cleanliness and hygiene habits in an elderly population. Aging Clin Exp Res 2005 Dec;17(6):502-507.
  9. Contemporary methods and mobile denture cleansers and theirs significance for older population. Med Pregl 2011 Sep-Oct;64(9-10):497-502.
  10. The prevalence of oral inflammation among denture wearing patients with chronic obstructive pulmonary disease. Adv Exp Med Biol 2015 Mar;858:87-91.
  11. 2-Amino-2methyl-1-propanol as the alkalizing agent in an improved continuous-flow cresolphtalein complexone procedure for calcium in serum. Clin Chem 1974 Nov;20(11):1458-1460.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.