International Journal of Experimental Dental Science

Register      Login

VOLUME 6 , ISSUE 2 ( July-December, 2017 ) > List of Articles

RESEARCH ARTICLE

Effect of Blood Contamination and Decontamination Procedures on the Microtensile Bond Strength of a New Self-etch Adhesive: An in vitro Study

Citation Information : Effect of Blood Contamination and Decontamination Procedures on the Microtensile Bond Strength of a New Self-etch Adhesive: An in vitro Study. Int J Experiment Dent Sci 2017; 6 (2):80-83.

DOI: 10.5005/jp-journals-10029-1161

License: CC BY 3.0

Published Online: 01-12-2010

Copyright Statement:  Copyright © 2017; The Author(s).


Abstract

Aim

The aim of the study was to evaluate the effect of blood contamination and decontamination procedures on the microtensile bond strength of a new self-etch adhesive before and after curing.

Materials and methods

A total of 90 human extracted mandibular molars were stored in 0.5% thymol solution and distilled water. Midcoronal sections were obtained using a diamond disk and the dentin surface was ground with 320 grit SIC abrasive paper. Universal self-etch adhesive (3M ESPC) and Filtex Z-250 resin composite were used. The dentin specimens were randomly divided into nine groups: Control group, group I—blood contamination before curing, group II—blood contamination before curing followed by air drying, group III—blood contamination before curing followed by rinsing with water and air drying, group IV—blood contamination before curing followed by rinsing with water, air dry, and reapplication of bonding agent, group V—blood contamination after curing, group VI—blood contamination after curing followed by air dry, group VII—blood contamination after curing followed by rinsing with water and air drying, group VIII—blood contamination after curing followed by rinsing with water, air dry, and reapplication of bonding agent. The microtensile bond strength was measured by universal testing machine and the data were analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test.

Results

The contamination groups (I, V) showed the least bond strength followed by the decontamination groups (II, III, VI, VII). The reapplication groups (IV, VIII) restored the bond strength equal to control group.

Clinical significance

A contamination-free area is required for adequate adhesion. It is important to rule out measures to prevent and manage contamination, so as to achieve durable seal between composite resin and tooth surface.

How to cite this article

Shaikh A, Hegde V, Shanmugasundaram S. Effect of Blood Contamination and Decontamination Procedures on the Microtensile Bond Strength of a New Self-etch Adhesive: An in vitro Study. Int J Experiment Dent Sci 2017;6(2):80-83.


HTML PDF Share
  1. Effect of blood contamination with 1 step self etch adhesives on microtensile bond strength to dentin. J Oper Dent 2006 Nov-Dec;31(6):660-665.
  2. Bonding efficiency and interfacial morphology of one bottle adhesive to contaminated dentin surfaces. Am J Dent 1998 Dec;11(6):281-285.
  3. Influence of sandwich class II restoration. J Oper Dent 2000 Sep-Oct;25(5):401-410.
  4. Influence of bond strength of self etching adhesive to dental tissues. J Adhes Dent 2011 Aug;13(4):349-358.
  5. Influence of blood contamination on bond strength between dentin and adhesive resin cement. Oper Dent 2000 May-Jun;25(3):195-201.
  6. The influence of the dentin smear layer on adhesion: a self-etching primer vs. a total-etch system. Dent Mater 2003 Dec;19(8):758-767.
  7. Bonding to dentin with a self-etching primer: the effect of smear layers. Dent Mater 2001 Mar;17(2):122-126.
  8. Effects of different burs on dentin bond strengths of self-etching primer bonding systems. Oper Dent 2001 Jul-Aug;26(4):375-382.
  9. Blood contamination and dentin bonding —effect of anticoagulant in laboratory studies. Dent Mater 2002 Mar;18(2):159-167.
  10. Effect of water, saliva and blood contamination on bonding of metal brackets with a GMETA/MMA/TBB resin to etched enamel. Am J Dent 1999 Dec;12(6):299-304.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.