International Journal of Experimental Dental Science

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2016 | July-December | Volume 5 | Issue 2

RESEARCH ARTICLE

SCIENCE TO SALES: DECODING THE COMPLEXITY OF RESEARCH

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:1] [Pages No:0 - 0]

PDF  |  DOI: 10.5005/ijeds-5-2-iv  |  Open Access |  How to cite  | 

RESEARCH ARTICLE

Regina TC Tandelilin

Oral Microbiome: The Microbial Gateway

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:1] [Pages No:0 - 0]

PDF  |  DOI: 10.5005/ijeds-5-2-vi  |  Open Access |  How to cite  | 

RESEARCH ARTICLE

Thuy Anh Vu Pham, Hao TT Nguyen, My TN Nguyen, Van NL Trinh, Nga Y Tran, Lan TQ Ngo, Ha LB Tran

Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:6] [Pages No:83 - 88]

PDF  |  DOI: 10.5005/jp-journals-10029-1131  |  Open Access |  How to cite  | 

Abstract

Aims

Our study focused on the fabrication of platelet-rich fibrin (PRF) and evaluated its influences on cell behaviors, including proliferation and migration.

Materials and methods

Platelet-rich fibrin was prepared from human peripheral blood according to Choukroun's method without using nonanticoagulant and foreign factors for platelet activation. Platelet-rich fibrin architecture was studied by hematoxylin and eosin staining. The investigation of PRF effects on human gingival fibroblasts (hGFs) was conducted via PRF liquid extract. Cell proliferation was determined via the number of cells after a period of time incubated in PRF liquid extract. Influence of PRF liquid extract on the migration of hGFs was conducted via scratch wound healing assay.

Results

Histological staining reviewed the natural fibrin fiber matrix of PRF. Platelet-rich fibrin liquid extract promoted hGF proliferation after 7 days of cultivation. Human gingival fibroblast proliferation in PRF liquid extract was more superior than those cultured in complete medium. Platelet-rich fibrin was also found to be able to promote the migration of hGFs for up to 48 hours.

Conclusion

These results indicated that PRF is suitable to be used as autologous natural biomaterial in supporting wound healing and in further application in periodontitis treatments.

How to cite this article

Nguyen HTT, Nguyen MTN, Trinh VNL, Tran NY, Ngo LTQ, Pham TAV, Tran HLB. Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts. Int J Experiment Dent Sci 2016;5(2):83-88.

RESEARCH ARTICLE

Manjiri Joshi, Mansi Tailor, Ajay Pala

Prevalence of Dental Caries, Periodontal Diseases, and Periapical Pathoses among Patients attending a Tertiary Dental Care Center in Central Gujarat: A Hospital-based Cross-sectional Study

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:4] [Pages No:89 - 92]

PDF  |  DOI: 10.5005/jp-journals-10029-1132  |  Open Access |  How to cite  | 

Abstract

Introduction

Dental caries and periodontal disease are the two globally leading oral infections. There is considerable variation in the prevalence of these diseases. Also, these may lead to various periapical pathoses like periapical abscess, cysts, and so on. These may be attributed to differences in age, gender, sample size, geographical distribution, and protocol followed for diagnosis.

Objectives

To estimate the prevalence of dental caries, gingivitis/periodontitis, and periapical pathoses in patients of all ages in a dental institute in central Gujarat.

Materials and methods

After obtaining approval from the Institutional Ethics Committee, a hospital-based cross-sectional study was carried out in a dental institute of central Gujarat. A total of 4,642 patients were screened, out of which 2,849 subjects were included in the present study. Intraoral examination was done under all aseptic conditions by using a diagnostic set of instruments by trained professionals and the status of the pathology recorded according to the standard procedure. Data were stored in an Excel spreadsheet for analysis.

Results

It was observed that dental caries/pulp pathoses were more prevalent in the younger age group with mean age 23.1 years. All the other pathoses were observed among the middle age group (above 35 years). Overall prevalence of dental caries/pulp pathoses, gingivitis/periodontitis, and periapical pathoses was observed in 54.8% of males as compared to 45.2% females.

Conclusion

The current study collected significant data for the prevalence of three different odontogenic oral lesions in the central Gujarat geographical location at a tertiary level. There is male preponderance in the prevalence of these diseases in general. There is a need to generate awareness about oral health and the prevention of dental caries and gingivitis/periodontitis and to institute measures for the provision of dental care services at the primary level.

How to cite this article

Joshi M, Tailor M, Pala A. Prevalence of Dental Caries, Periodontal Diseases, and Periapical Pathoses among Patients attending a Tertiary Dental Care Center in Central Gujarat: A Hospital-based Cross-sectional Study. Int J Experiment Dent Sci 2016;5(2):89-92.

RESEARCH ARTICLE

Alberto Dagna, Riccardo Beltrami, Stefano Bianchi, Claudio Poggio, Giulia Gastaldo

Scanning Electron Microscopic Evaluation of Root Canal Walls after shaping with Different Single-use Rotary Systems

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:6] [Pages No:93 - 98]

PDF  |  DOI: 10.5005/jp-journals-10029-1133  |  Open Access |  How to cite  | 

Abstract

How to cite this article

Dagna A, Gastaldo G, Beltrami R, Bianchi S, Poggio C. Scanning Electron Microscopic Evaluation of Root Canal Walls after shaping with Different Single-use Rotary Systems. Int J Experiment Dent Sci 2016;5(2):93-98.

RESEARCH ARTICLE

Karmel V Headen, Afolabi O Ogunleye, David E Williams

A High-resolution Immunohistochemical Method for studying Receptor Expression on the Periodontal Ligament of Whole-mount Human Tooth Roots

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:5] [Pages No:99 - 103]

PDF  |  DOI: 10.5005/jp-journals-10029-1134  |  Open Access |  How to cite  | 

Abstract

Aims

Our laboratory has found that lysophosphatidic acid (LPA) and its cognate receptors [LPARs, (LPA1–6)] expressed by human gingival fibroblasts (GF) and periodontal ligament fibroblasts (PDLF) play key roles in oral fibroblast homeostasis and are implicated in the inflammation seen in periodontal disease. We have reported that PDLF express LPA1 and LPA3; however, information on the gross topographic distribution of LPARs in the periodontal ligament (PDL) was lacking, and therefore, we developed a simple method for in situ labeling of LPARs in the PDL of extracted teeth.

Materials and methods

Sectioning or grinding thin sections of demineralized or native teeth and periodontium have long been the standard methodologies used to assess biomarker distribution in the PDL; however, we modified traditional immunohistochemical labeling and used whole teeth with fixed, solvent permeabilized PDLs.

Results

LPA1 and LPA3 were specifically labeled in the PDL and could be visualized at both the macroand micro-levels.

Conclusion

This technique effectively labeled LPARs, and it can serve as a basis for the in situ visualization of other biomolecules expressed in the PDL.

Clinical Significance

The ability to observe PDL LPAR distribution at the macro-level complements the microscopic data, and it is useful for detecting and documenting molecular changes in the PDL/PDLF that were brought about by age, experimental treatments, or pathologies like periodontal disease.

How to cite this article

Cerutis DR, Headen KV, Ogunleye AO, Williams DE. A High-resolution Immunohistochemical Method for studying Receptor Expression on the Periodontal Ligament of Whole-mount Human Tooth Roots. Int J Experiment Dent Sci 2016;5(2):99-103.

RESEARCH ARTICLE

Franklin Garcia-Godoy, Mohammad Ali Saghiri, Jafar Orangi, Nader Tanideh, Armen Asatourian, Nader Sheibani

Influence of Additives to Bovine Bone Material in Osseous Regeneration of Mandibular Defect: An Animal Study using DXA

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:5] [Pages No:104 - 108]

PDF  |  DOI: 10.5005/jp-journals-10029-1135  |  Open Access |  How to cite  | 

Abstract

Introduction

Increasing bone quality and quantity in the areas with insufficient bone volume is a major concern among scientists. Ideal bone substitute materials should have osteogenicity, osteoconductivity, and osteoinductivity. Clinoptilolite offers bovine deorganified crystalline bone materials, the advantage of being very similar to human bone with regard to its pore morphology and crystalline structure. This study evaluated the effect of adding Clinoptilolite to Bio-Oss on the osseous regeneration and bone healing process using serial dual-energy X-ray absorptiometry (DXA).

Materials and methods

A total of 64 rabbits were anesthetized and a bone defect was created on both semi-mandibles. The rabbits were divided into four equal groups: A (Bio-Oss®); B (Bio-Oss® with 2% Clinoptilolite mixture); C (allograft); and D receiving no treatment. The bone healing response of animals was tested after 2, 14, 30, and 60 days.

Results

Statistical analysis showed significant differences at time intervals before 14 days between allograft and other groups (p < 0.05). In all the defects filled with the tested materials, bone formation was observed subjectively. At 30-and 60-day intervals, there were no significant differences between allograft and Bio-Oss with 2% Clinoptilolite group (p = 0.052 and p = 0.260 respectively) although it was significant in 2-and 14-day intervals.

Conclusion

Clinoptilolite (2%) can be used to improve the osteoinduction property of bovine deorganified crystalline bone material. Clinoptilolite can be suggested as a potential material added to bone substitute materials due to its porous structure and buffering capacity and adsorption of a number of serum components which aids the osseous regeneration and healing process.

How to cite this article

Saghiri MA, Orangi J, Tanideh N, Asatourian A, Garcia-Godoy F, Sheibani N. Influence of Additives to Bovine Bone Material in Osseous Regeneration of Mandibular Defect: An Animal Study using DXA. Int J Experiment Dent Sci 2016;5(2):104-108.

RESEARCH ARTICLE

Kamran Bokhari, Abdul A Shahrani, Abdel B Mustafa, Yhya Hdban, Mohammad Saleh, Abdullah Mofareh

Position of Mental Foramen among Saudi Population: A Radiographic Study

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:4] [Pages No:109 - 112]

PDF  |  DOI: 10.5005/jp-journals-10029-1136  |  Open Access |  How to cite  | 

Abstract

How to cite this article

Bokhari K, Shahrani AA, Mustafa AB, Hdban Y, Saleh M, Mofareh A. Position of Mental Foramen among Saudi Population: A Radiographic Study. Int J Experiment Dent Sci 2016;5(2):109-112.

RESEARCH ARTICLE

Sara Rayner, Trislyn Herrick, Christina Chiesa

Efficacy of “Oil Pulling” on Gingival Health and Biofilm Accumulation in a Group of Young Adults: A Pilot Study

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:5] [Pages No:113 - 117]

PDF  |  DOI: 10.5005/jp-journals-10029-1137  |  Open Access |  How to cite  | 

Abstract

Aims

The aim of this pilot study was to assess changes in gingival bleeding and biofilm accumulation in young adults undergoing oil-pulling treatment together with standardized oral hygiene practices, compared to a control group of patients that did not undergo oil pulling but maintained the standard oral hygiene practices outlined in the study.

Materials and methods

The experiment was designed as a randomized, two-way, single-blind, crossover with a 2-month washout period between two, 10-day experimental periods. A total of 28, randomly selected dental students who met the inclusion criteria were assessed for gingival bleeding and biofilm on six teeth before and after rinsing with sesame oil for 10 minutes per day, for a period of 10 days. The mean difference between the test and control group's reduction in scores was analyzed using the paired t-test. The level of significance was set at ? (alpha) = 0.05.

Results

There were no statistically significant differences on plaque biofilm accumulation and bleeding when compared with the control group. There was a higher reduction in gingival bleeding scores (albeit not statistically significant) among those who used sesame oil compared to those who did not.

Conclusion

Based on the current study, it appears that oil pulling for 10 minutes and for a period of 10 days using sesame oil has no significant benefit over brushing and flossing alone, on a sample of 28 dental students.

How to cite this article

Rayner S, Herrick T, Chiesa C, Hoover J, Karunanayake C. Efficacy of “Oil Pulling” on Gingival Health and Biofilm Accumulation in a Group of Young Adults: A Pilot Study. Int J Experiment Dent Sci 2016;5(2):113-117.

RESEARCH ARTICLE

Mohammed Jasim Aljuboori, Hussien Ali Al-Wakeel, Lor Yen Fang, Navenithamaria Eirutharajan, Santhanalaxmi A Balachandran

Evaluation of the Intermental Foramina Distance among Malaysian Population by using Orthopantomogram Radiograph

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:5] [Pages No:118 - 122]

PDF  |  DOI: 10.5005/jp-journals-10029-1138  |  Open Access |  How to cite  | 

Abstract

Objectives

Previous studies showed there were variations in the interforamina distance of mental foramen between races. The aim of our study is to determine the interforamina distance of mental foramen among Malaysian populations.

Materials and methods

A total of 503 orthopantomogram (OPG) radiographs were observed. Interexaminer calibration was estimated using the Kappa-Cohen formula. A sheet of transparent paper was superimposed on the panoramic film and the mental foramen was traced on it by using a fine marker pen. A vertical line marking the midline was drawn passing between the maxillary central incisors, the mandibular central incisors, or the nasal septum by using a ruler. The distance of the mental foramen from the midline was then recorded from both the left and the right side. The data were analyzed using chi-square test.

Results

According to our inclusion and exclusion criteria, 110 OPGs have been excluded and 393 OPGs were selected. In the Malay population, the most common interforamina distance for a male is between 55 and 60+ mm (n= 73, 54.8%). Also, the most common interforamina distance for a female is 55 to 60+ mm (n = 84, 50%). In the Chinese population, the most common interformina distance for a male is between 55 and 60+ mm (n=60, 66.8%). Similarly, the most common interforamina distance for a female is between 55 and 60+ mm (n= 73, 53.8%). Among the Indian population, the most common interforamina distance for a male falls between 55 and 60+ mm (n= 53, 43.4%) and for a female as well (n=50, 54%). According to the chi-square test, the results show that the study is statistically insignificant since the p-value is >0.05 for both the male and female population.

Conclusion

There is no relationship between the interforamina distance and the ethnicity. The most common interforamina distance for all the races falls between 55 and 60+ mm.

How to cite this article

Al-Juboori MJ, Saini R, Al-Wakeel HA, Fang LY, Eirutharajan N, Balachandran SA. Evaluation of the Intermental Foramina Distance among Malaysian Population by using Orthopantomogram Radiograph. Int J Experiment Dent Sci 2016;5(2):118-122.

RESEARCH ARTICLE

Tiago N Pinheiro, Juliana Arid, Aloizio P Maciel, André FM Machado, Marleno L Monteiro, Mariana de Oliveira Daltoé

Acute Suppurative Sialadenitis Secondary to Excisional Ranula Biopsy in Child

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:4] [Pages No:123 - 126]

PDF  |  DOI: 10.5005/jp-journals-10029-1139  |  Open Access |  How to cite  | 

Abstract

Introduction

Ranulas are lesions that affect the salivary glands, and they are generally treated by excision; however, they can recur in some cases where the gland is not removed and they may progress to acute suppurative sialadenitis, yet such cases are rare, especially in children.

Aims

To report clinical management of occurrence of acute suppurative sialadenitis secondary to excisional biopsy of ranula in a child.

Case report

An 11-year-old female patient was diagnosed with ranula where excisional biopsy was performed. Subsequently, the patient developed infection, suggesting Ludwig's angina, after reevaluation, she was diagnosed with acute suppurative sialadenitis in the right mandibular gland; during the follow-up period, antibiotic therapy was introduced.

Conclusion

The close relationship of the biopsy site with the submandibular space and other structures of the neck required the completion of antibacterial prophylaxis associated with preoperative care, transoperative care, and postoperative care to combat opportunistic infections and their subsequent complications.

Clinical Significance

Suppurative sialadenitis and its subsequent complications may be considered, although rare, as a complication in cases of ranula.

How to cite this article

Pinheiro TN, Arid J, Maciel AP, Machado AFM, Monteiro ML, de Oliveira Daltoé M, de Carvalho FK. Acute Suppurative Sialadenitis Secondary to Excisional Ranula Biopsy in Child. Int J Experiment Dent Sci 2016;5(2):123-126.

RESEARCH ARTICLE

WKL Perera, EAAP Amaratunga, R Sivakanesan

Effects of Periodontal Disease and Periodontal Treatment on the Lipid Profile: A Sri Lankan Study

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:6] [Pages No:127 - 132]

PDF  |  DOI: 10.5005/jp-journals-10029-1140  |  Open Access |  How to cite  | 

Abstract

Aims

To compare the blood lipid levels of periodontitis group (of different severities) with a nonperiodontitis group and to evaluate any influence of periodontal treatment in altering blood lipid levels.

Materials and methods

The study sample consisted of 83 patients with periodontitis in the “test” group and a matched “control” group of 40, free of periodontitis according to predetermined selection criteria. All subjects underwent periodontal assessments and blood lipid estimation prior to treatment planning, following which they received active periodontal treatment/oral prophylaxis. Twelve weeks after treatment, periodontal reevaluations were carried out and blood lipids were reassessed for preand posttreatment comparisons.

Results

Increases in lipid levels were observed in the periodontitis group compared to the nonperiodontitis group at baseline. The test group had 5% higher mean total cholesterol than the control group at pretreatment/preintervention (albeit not significant: Student's t), while low-density lipoprotein cholesterol (LDL-Ch) was 6.6% higher in the test group than the control group (95% confidence interval). Mean triglycerides (Tri-G) were 5.7% higher in the control group than the test group, yet without statistical significance. All types of lipids in both the groups had decreased following periodontal therapy, while Tri-G levels demonstrated an opposite effect.

Conclusion

Patients with generalized severe periodontitis had higher LDL-Ch values than those with generalized mildmoderate periodontitis. Periodontal care was effective in lowering unfavorable serum lipids. Prompt control of periodontal inflammation would minimize development of deep periodontal infection and cardiovascular risk.

How to cite this article

Tilakaratne A, Perera WKL, Amaratunga EAAP, Sivakanesan R. Effects of Periodontal Disease and Periodontal Treatment on the Lipid Profile: A Sri Lankan Study. Int J Experiment Dent Sci 2016;5(2):127-132.

Source of support

University of Peradeniya Sri Lanka (Research Grant Number RG/2009/40/D).

RESEARCH ARTICLE

Jian-hong YU, Ya-Yu Tsai, Chih-Yu Fang

Improved Superelastic NiTi wire for the Treatment of Adult Skeletal Class III Malocclusion in a Surgery-first Case

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:6] [Pages No:133 - 138]

PDF  |  DOI: 10.5005/jp-journals-10029-1141  |  Open Access |  How to cite  | 

Abstract

How to cite this article

Fang C-Y, Tsai Y-Y, Yu J-H. Improved Superelastic NiTi wire for the Treatment of Adult Skeletal Class III Malocclusion in a Surgery-first Case. Int J Experiment Dent Sci 2016;5(2):133-138.

RESEARCH ARTICLE

Tsz M Leung, Michael G Botelho, Yanqi Yang, Edward HT Ho

Management of Tooth Wear: An Interdisciplinary Approach

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:7] [Pages No:139 - 145]

PDF  |  DOI: 10.5005/jp-journals-10029-1142  |  Open Access |  How to cite  | 

Abstract

Aims

This case report aims to describe a conservative interdisciplinary approach to restore generalized tooth wear complicated with malalignment of teeth.

Introduction

Tooth wear is the surface loss of dental hard tissue, i.e., usually multifactorial in origin, excluding other than caries, trauma, or developmental disorder. Severe tooth wear impact the appearance, comfort, function, or longevity of the tooth. Prosthodontic management of patients with tooth wear has been well documented, although prosthodontic treatment is designed to correct minor malocclusion, patients with moderate-to-severe malocclusion accompanied by generalized tooth wear may require preprosthetic orthodontic intervention.

Case report

This case report describes a 47-year-old male patient with generalized tooth wear complicated with lack of interocclusal space and malalignment of teeth. Preventive measures were carried out to address the etiological factors of tooth wear. After orthodontic treatment, definitive restoration was placed in reorganized occlusion at an increased vertical dimension, which restored esthetic and function.

Conclusion

In this clinical report, generalized tooth wear with insufficient space for restoration, complicated by malalignment of teeth, was managed by way of an interdisciplinary approach which allowed a conservative full-mouth rehabilitation. After orthodontic treatment, a reorganized restorative approach was undertaken at an increased vertical dimension, providing an esthetic and functional solution. An occlusal night guard was provided to protect the dental restorations.

Clinical Significance

Interdisciplinary approach allows a conservative management for patient with tooth wear complicated by malalignment of teeth.

How to cite this article

Leung TM, Botelho MG, Yang Y, Ho EHT. Management of Tooth Wear: An Interdisciplinary Approach. Int J Experiment Dent Sci 2016;5(2):139-145.

RESEARCH ARTICLE

Priyanka Jain, Brajendra S Tomar, GS Chandu, Shubhra Singh, Monika Khatri

Concealment of Amputation: Silicone Finger Prosthesis

[Year:2016] [Month:July-December] [Volume:5] [Number:2] [Pages:5] [Pages No:146 - 150]

PDF  |  DOI: 10.5005/jp-journals-10029-1143  |  Open Access |  How to cite  | 

Abstract

How to cite this article

Tomar BS, Chandu GS, Singh S, Sapat M, Khatri M, Jain P. Concealment of Amputation: Silicone Finger Prosthesis. Int J Experiment Dent Sci 2016;5(2):146-150.

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